Primary structure of Escherichia coli glutamine synthetase. 3. Chemical evidence for subunit identity; amino acid sequences at the NH 2 and COOH termini.
نویسندگان
چکیده
Evidence that the glutamine synthetase dodecamer from Escherichia coli is comprised of 12 identical subunits of molecular weight 49,000 has been obtained by end group analysis. Application of the carbamylation procedure to the NH*terminal analysis revealed only serine. The yield was 98% of that expected from a polypeptide of the size proposed for the monomeric species. The following partial sequence of residues at the NH2 terminus of the molecule was elucidated with the aid of automated sequencing equipment:
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 247 24 شماره
صفحات -
تاریخ انتشار 1972